Single Cell Full Length mRNA-Amplification Kit
Catalog No: IMSA-02005
Size:
12 reactions;24 reactions;96 reactions
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| Product Detail |
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| Description |
Single Cell Full Length mRNA Amplification Kit can synthesize cDNA using 1-500 cells or 10 pg-10 ng of total RNA as template and Oligo(dT) VN Primer as reverse transcription primer, and use the Template-switching activity of Reverse Transcriptase Sc The cDNA was synthesized using Oligo(dT) VN Primer as the reverse transcription primer, and the reverse transcriptase Sc Reverse-switching activity was used to add a splice sequence at the 3' end of the cDNA, through which subsequent PCR amplification was performed to obtain the full-length cDNA amplification product. The full-length cDNA amplification product can be sequenced and analyzed to obtain gene expression differences, variable splicing, fusion genes and other genetic regulatory information. |
| Shipping Condition |
Dry ice |
| Component |
5’ TS Oligo Primer; Store at -80 °C
Control Total RNA (1 μg/μl); Store at -80 °C
Lysis Buffer; Store at -20 °C
RNase Inhibitor; Store at -20 °C
Oligo (dT) VN Primer; Store at -20 °C
dNTP Mix; Store at -20 °C
DTT; Store at -20 °C
1st Strand Buffer; Store at -20 °C
Sc Reverse Transcriptase; Store at -20 °C
2 × Amplification Mix
PCR Primer
Elution Buffer
Nuclease-free ddH2O |
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