T-Cell Protein Profiling by CyTOF

Recent applications of mass spectrometry (CyTOF) in biology have provided a "systems" approach to monitor simultaneous changes in multiple host cytokines at the single cell level. CD Genomics offers T cell protein profiling services by CyTOF, helping our clients to document phenotypic and functional complexity and changes in T cells.

Why Do We Study T-cell Protein Profiles?

T cells consist of a heterogeneous group of functionally distinct lymphocytes and their immune function is mainly to fight intracellular infections, tumor cells, and allogeneic cells. T cells can produce a delayed allergic response, and this specific immune response by T lymphocytes is called cytosolic immunity. The analysis of T cell protein profiles can be used to reveal the influence of targeted differentiated cells in the pathogenesis of multiple diseases, including their surface characteristics and the signaling pathways that regulate these characteristics. This facilitates the understanding of disease processes and the study of potential diagnostic and therapeutic approaches.

Applications of CyTOF in T-Cell Protein Profiling

Unlike standard flow cytometry methods using fluorophore-linked Abs, mass spectrometry avoids the limitations associated with spectral overlap and can theoretically detect more than 40 parameters per cell, providing higher resolution at the single-cell level.

• CyTOF Profiles Human T-Cell Exhaustion
  In contrast to typical functional T cell subsets such as naive T cells (T_N), effector T cells (T_EFF) and memory T cells (T_MEM), failing T cells (T_EX) exhibit substantial functional, transcriptomic and epigenomic differences. T_EX is a unique T cell population with a critical role in many chronic infections and cancers. Therefore, accurate high-dimensional parametric analysis of T_EX using mass spectrometry can help to better understand the role of T_EX in chronic infections and cancer. It has important implications for immune monitoring in therapeutic settings aimed at improving T cell immunity, for example during cancer immunotherapy.

Fig.1 A systematic immunological approach to the characterization of T_EX using mass spectrometry. (Winkler, F., & Bengsch, B., 2020)

Our Services

Mass spectrometry has become a transformative technology for human immune cell analysis. Using purified metal isotopes as labels for specific antibodies to stain individual cells and detecting these labeled isotopes on ionized cells by time-of-flight mass spectrometry, protein expression of over 40 insightful markers on individual cells can be analyzed.

CD Genomics is a precursor in the application of mass spectrometry platforms for exploratory research projects and clinical sample analysis. We provide highly customizable T cell protein profiling services to simultaneously obtain information on single cell surface markers, intracellular signaling pathways, transcription factors, cytokines, cell cycle, and so on, for a wide range of applications in hematopoietic, immune, stem cell, cancer, and drug screening research.

• Multiple Markers
  We can reach 40 markers, identify several cell subpopulations simultaneously, and characterize intracellular targets in a single sample.
• Multiple Mass Cytometry Abs
  We design the most ideal antibody Panel for your project. We have an available library of metal-bonded Abs. In addition, we can provide custom antibody labeling services and can also label purified antibodies that you provide.
• Data Analysis
  Our professional data analysis team provides efficient and reliable viSNE analysis services, a tool for mapping high-dimensional mass cytometry data to two dimensions while conserving high-dimensional structure, and comparative analysis services.

Workflow of T-Cell Protein Profiling by CyTOF

Why Choose Us

CD Genomics' mass spectrometry flow-through systems have extremely high signal resolution and can detect multiple different labels at the same time, solving bottlenecks for our customers' immunomics projects. Please contact us for more information.

Reference

1. Winkler, F., & Bengsch, B. (2020). Use of mass cytometry to profile human T cell exhaustion. Frontiers in immunology, 10, 3039.

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